Anti-Chk1 (phospho Ser345) Antibody [ARC56803] (A308650)

Name: Anti-Chk1 (phospho Ser345) Antibody [ARC56803]
Brand: Antibodies.com
SKU: A308650
Price: 540.0 USD
Availability: InStock

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$540

Rabbit monoclonal [ARC56803] antibody to Chk1 (phospho Ser345) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Rabbit polyclonal antibody to Chk1 for WB and ELISA.

Specifications

Name

Anti-Chk1 (phospho Ser345) Antibody [ARC56803]

Description

Rabbit monoclonal [ARC56803] antibody to Chk1 (phospho Ser345).

Applications

Dilutions

WB: 1:1,000-1:5,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S345 of human Phospho-Chk1-S345 (NP_001107593.1).

Sequence

SFSQP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC56803

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

56 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C85740, Cell cycle checkpoint kinase, Checkpoint , S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, CHK1 checkpoint homolog (S. pombe), CHK1_HUMAN, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Chk1 (phospho Ser345) Antibody [ARC56803] (A308650)

Western blot analysis of various lysates, using Anti-Chk1 (phospho Ser345) Antibody [ARC56803] (A308650) at 1:2,000 dilution. HeLa cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Chk1 (phospho Ser345) Antibody [ARC56803] (A308650)

Western blot analysis of various lysates, using Anti-Chk1 (phospho Ser345) Antibody [ARC56803] (A308650) at 1:2,000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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