Anti-Chk1 (phospho Ser345) Antibody (A11056)

Name: Anti-Chk1 (phospho Ser345) Antibody
Brand: Antibodies.com
SKU: A11056
Price: 435.0 USD
Availability: InStock

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$435

Rabbit polyclonal antibody to Chk1 (phospho Ser345) for WB.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Anti-Chk1 Antibody

Rabbit polyclonal antibody to Chk1 for WB and ELISA.

Specifications

Name

Anti-Chk1 (phospho Ser345) Antibody

Description

Rabbit polyclonal antibody to Chk1 (phospho Ser345).

Applications

Dilutions

WB: 1:500-1:2,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S345 of human Chk1 (NP_001265.2).

Sequence

SFSQP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

54 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C85740, Cell cycle checkpoint kinase, Checkpoint , S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chek1, Chk 1, CHK1 checkpoint homolog (S. pombe), CHK1_HUMAN, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Chk1 (phospho Ser345) Antibody (A11056)

Western blot analysis of extracts of various cell lines, using Anti-Chk1 (phospho Ser345) Antibody (A11056). The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.

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Figure 2: Western Blot - Anti-Chk1 (phospho Ser345) Antibody (A11056)

Western blot analysis of extracts of C6 cells, using Anti-Chk1 (phospho Ser345) Antibody (A11056) at 1:1,000 dilution or Chk1 antibody (A7653). C6 cells were treated by ATP(5 mM) at 30°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5min.

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