Anti-Chromogranin A Antibody (A84217)

Name: Anti-Chromogranin A Antibody
Brand: Antibodies.com
SKU: A84217
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Chromogranin A for ELISA, WB and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Chromogranin A Antibody

Description

Goat polyclonal antibody to Chromogranin A.

Applications

ELISA, WB, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.3-1µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human Chromogranin A.

Sequence

C-QGAKERAHQQKKH

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

50.7 kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

beta Granin, betagranin (N-terminal fragment of chromogranin A), catestatin, CgA, CHG A, Chga, chromofungin, Chromogranin A parathyroid secretory protein 1, Chromogranin A precursor, ChromograninA, CMGA_HUMAN, ER-37, Pancreastatin, Parastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Secretory protein I, SP I, SP-I, SP1, SPI, Vasostatin, Vasostatin I, Vasostatin II

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Chromogranin A Antibody (A84217)

Chromogranin A expression in Jurkat (A) and LNCaP (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Chromogranin A Antibody (A84217) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-Chromogranin A Antibody (A84217)

Chromogranin A expression in Human Cerebellum (A), Kidney (B), Pancreas iso 1 (C) and Pancreas iso 2 (D) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-Chromogranin A Antibody (A84217) at 0.3µg/ml (A) or 1µg/ml (B-D) and detected by chemiluminescence.

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Figure 3: Flow Cytometry - Anti-Chromogranin A Antibody (A84217)

Chromogranin A expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Chromogranin A Antibody (A84217) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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