Anti-CHEK1 (Phospho-Ser317) Antibody (A50557)

Name: Anti-CHEK1 (Phospho-Ser317) Antibody
Brand: Antibodies.com
SKU: A50557
Price: 375.0 USD
Availability: InStock

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$375

Rabbit polyclonal antibody to CHEK1 (Phospho-Ser317) for WB and IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-CHEK1 (Phospho-Ser317) Antibody

Description

Rabbit polyclonal antibody to CHEK1 (Phospho-Ser317)

Specificity

The antibody detects endogenous level of CHEK1 only when phosphorylated at serine 317.

Applications

WB, IF

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S317 of human CHEK1.

Host

Rabbit

Clonality

Polyclonal

Conjugate

Unconjugated

Purification

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1.0mg / ml

Formulation

Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store at -20˚C

Synonyms

C85740, Cell cycle checkpoint kinase, Checkpoint , S. pombe, homolog of, 1, Checkpoint kinase 1, Checkpoint kinase 1 homolog (S. pombe), CHEK 1, Chk 1, Chk1, CHK1 checkpoint homolog (S. pombe), CHK1_HUMAN, EC 2.7.11.1, rad27, Serine/threonine protein kinase Chk1, Serine/threonine-protein kinase CHK1, STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Anti-CHEK1 (Phospho-Ser317) Antibody (A50557)

Western blot analysis of extracts of MCF7 cell line, using Phospho-CHEK1-S317 antibody.

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Figure 2: Immunofluorescence - Anti-CHEK1 (Phospho-Ser317) Antibody (A50557)

Immunofluorescence analysis of MCF7 cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.

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Figure 3: Immunofluorescence - Anti-CHEK1 (Phospho-Ser317) Antibody (A50557)

Immunofluorescence analysis of U2OS cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

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