Anti-CHCHD2 Antibody (A88474)

Name: Anti-CHCHD2 Antibody
Brand: Antibodies.com
SKU: A88474
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to CHCHD2 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-CHCHD2 Antibody

Description

Rabbit polyclonal antibody to CHCHD2.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1).

Sequence

TLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

16.7kD protein, Aging Associated Gene 10 Protein, Aging-associated gene 10 protein, C7orf17, CHCH2_HUMAN, CHCHD 2, Coiled Coil Helix Coiled Coil Helix Domain Containing 2, Coiled coil helix coiled coil helix domain containing protein 2, mitochondrial, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, HCV NS2 trans regulated protein, HCV NS2 trans-regulated protein, NS2TP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-CHCHD2 Antibody (A88474)

Western blot analysis of extracts of various cell lines, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-CHCHD2 Antibody (A88474)

Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using Anti-CHCHD2 Antibody (A88474) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-CHCHD2 Antibody (A88474)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-CHCHD2 Antibody (A88474) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.