Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CENP A, CENP-A, CENPA_HUMAN, Centromere autoantigen A, Centromere protein A, Centromere protein A 17kDa, Histone H3 like centromeric protein A, Histone H3-like centromeric protein A
Figure 1: Western Blot - Anti-CENPA Antibody (A88437)
Western blot analysis of extracts of various cell lines, using Anti-CENPA Antibody (A88437) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Immunofluorescence analysis of U-2 OS cells using Anti-CENPA Antibody (A88437) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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