Anti-Cdk9 Antibody [ARC0527] (A81021)

Name: Anti-Cdk9 Antibody [ARC0527]
Brand: Antibodies.com
SKU: A81021
Price: 470.0 USD
Availability: InStock

Enlarge Image

$470

Rabbit monoclonal [ARC0527] antibody to Cdk9 for WB, IHC, ICC/IF, IP, ChIP and ChIP-seq.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Cdk9 Antibody [ARC0527]

Description

Rabbit monoclonal [ARC0527] antibody to Cdk9.

Applications

WB, IHC, ICC/IF, IP, ChIP, ChIP-seq

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:50-1:200, ChIP-seq: 2-4µg/IP, CUT&Tag: 1⁶ cells/5µg

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 273-372 of human CDK9 (P50750).

Sequence

RKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0527

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

42 kDa / 55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C-2K, CDC2 related kinase, CDC2L4, Cdk 9, CDK9_HUMAN, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, CTK1, Cyclin dependent kinase 9, Cyclin-dependent kinase 9, PITALRE, Serine/threonine-protein kinase PITALRE, TAK, Tat associated kinase complex catalytic subunit

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

Enlarge Image

Figure 1: Anti-Cdk9 Antibody [ARC0527] (A81021)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 5µg of Anti-Cdk9 Antibody [ARC0527] (A81021), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of CDK9 in representative gene loci (RAC1), as shown in figure.

Enlarge Image

Figure 2: Western Blot - Anti-Cdk9 Antibody [ARC0527] (A81021)

Western blot analysis of extracts of various cell lines, using Anti-Cdk9 Antibody [ARC0527] (A81021) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

Enlarge Image

Figure 3: Western Blot - Anti-Cdk9 Antibody [ARC0527] (A81021)

Western blot analysis of extracts from wild type(WT) and CDK9 knockout (KO) 293T cells, using Anti-Cdk9 Antibody [ARC0527] (A81021) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-Cdk9 Antibody [ARC0527] (A81021)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Cdk9 Antibody [ARC0527] (A81021) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.