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Anti-CDK5RAP1 Antibody (A90897)

Western Blot - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.com Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.com Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.com Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CDK5RAP1 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-CDK5RAP1 Antibody
Description
Rabbit polyclonal antibody to CDK5RAP1.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CDK5RAP1 (NP_057492.2).
Sequence
MHPLQCVLQVQRSLGWGPLASVSWLSLRMCRAHSSLSSTMCPSPERQEDGARKDFSSRLAAGPTFQHFLKSASAPQEKLSSEVEDPPPYLMMDELLGRQRKVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQEADVILLVTCSIREKAEQTIWNRLHQLKALKTRRPRSRVPLRIGILGCMAERLKEEILNREKMVDILAGPDAYRDLPRLLAVAESGQQAANVLLSLDETYADVMPVQTSASATSAFVSIMRGCDNMCSYCIVPFTRGRERSRPIASILEEVKKLSEQGLKEVTLLGQNVNSFRDNSEVQFNSAVPTNLSRGFTTNYKTKQGGLRFAHLLDQVSRV
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
68 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
2310066P17Rik, C20orf34, C42, CDK5 activator binding protein C42, CDK5 activator binding protein C42 like, CDK5 regulatory subunit associated protein 1, CDK5RAP1.3, CDK5RAP1.4, CGI 05, HSPC167, OTTMUSP00000016976, RP23-154J12.6, RP5-1187J4.1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CDK5RAP1 Antibody (A90897)
Western blot analysis of extracts of various cell lines, using Anti-CDK5RAP1 Antibody (A90897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 30s.
Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897)
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-CDK5RAP1 Antibody (A90897) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CDK5RAP1 Antibody (A90897) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CDK5RAP1 Antibody (A90897)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-CDK5RAP1 Antibody (A90897) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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