This antibody recognizes an extracellular epitope of CD318, a type I transmembrane protein involved in early hematopoiesis.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
NIH-3T3/CD318 cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
CUB1
Isotype
IgG2b
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD318, CUB domain-containing protein 1, Membrane glycoprotein gp140, SIMA135, Subtractive immunization M plus HEp3-associated 135 kDa protein, Transmembrane and associated with src kinases, TRASK
Separation of HT-29 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using Anti-CD318 Antibody (APC) (10 µl reagent per milion cells in 100 µl of cell suspension).
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