Anti-Cdc25C Antibody [ARC0622] (A307870)

Name: Anti-Cdc25C Antibody [ARC0622]
Brand: Antibodies.com
SKU: A307870
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0622] antibody to Cdc25C for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Cdc25C Antibody [ARC0622]

Description

Rabbit monoclonal [ARC0622] antibody to Cdc25C.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cdc25C (P30307).

Sequence

MSTELFSSTREEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCPDVPRTPVGKFLGDSANLSILSGGTPKRCLDLSNLSSGEITATQLTTSADLDETGHLD

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0622

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 - 65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDC 25, Cdc 25C, CDC25, Cell division cycle 25 homolog C, Cell division cycle 25C, Cell division cycle 25C protein, Dual specificity phosphatase Cdc25C, M phase inducer phosphatase 3, M-phase inducer phosphatase 3, Mitosis inducer CDC25, MPIP3, MPIP3_HUMAN, Phosphotyrosine phosphatase, PPP1R60, protein phosphatase 1, regulatory subunit 60

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Cdc25C Antibody [ARC0622] (A307870)

Western blot analysis of extracts of various cell lines, using Anti-Cdc25C Antibody [ARC0622] (A307870) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunofluorescence - Anti-Cdc25C Antibody [ARC0622] (A307870)

Immunofluorescence analysis of HeLa cells using Anti-Cdc25C Antibody [ARC0622] (A307870) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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