Anti-Cdc25B Antibody [ARC1736] (A308797)

Name: Anti-Cdc25B Antibody [ARC1736]
Brand: Antibodies.com
SKU: A308797
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1736] antibody to Cdc25B for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Cdc25B Antibody [ARC1736]

Description

Rabbit monoclonal [ARC1736] antibody to Cdc25B.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CDC25B (P30305).

Sequence

SSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1736

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI604853, Cdc 25B, CDC25HU2, Cdc25m2, Cell division cycle 25 homolog B, Cell division cycle 25B, Cell division cycle 25B isoform 1, Cell division cycle 25B isoform 2, Cell division cycle 25B isoform 3, Cell division cycle 25B isoform 4, Cell division cycle 25B isoform 5, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M phase inducer phosphatase 2, M-phase inducer phosphatase 2, MPIP2_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Cdc25B Antibody [ARC1736] (A308797)

Western blot analysis of extracts of various cell lines, using Anti-Cdc25B Antibody [ARC1736] (A308797) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-Cdc25B Antibody [ARC1736] (A308797)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-Cdc25B Antibody [ARC1736] (A308797) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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