Anti-CD99 Antibody [SPM586] (A251051)

Mouse monoclonal [SPM586] antibody to CD99 for Flow Cytometry, IF and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD99 Antibody [SPM586]
Description
Mouse monoclonal [SPM586] antibody to CD99.
Specificity
This antibody recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Applications
Flow Cytometry, IF, IHC-P
Dilutions
Flow Cytometry: 5-10 µl / million cells, IF: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human, Rat
Immunogen
Purified E-rosette forming cells from human peripheral blood lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
SPM586
Isotype
IgM
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
27-32 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD99 Antibody [SPM586] - BSA and Azide free (A254328).
Synonyms
12E7, Antigen identified by monoclonal 12E7, Y homolog, Antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigen, CD99 molecule, CD99_HUMAN, Cell surface antigen 12E7, Cell surface antigen HBA 71, Cell surface antigen O13, E2 antigen, HBA71, MIC 2X, MIC 2Y, MIC2, MIC2 (monoclonal, MIC2X, MIC2Y, MSK5X, Protein MIC2, Surface antigen MIC2, T cell surface glycoprotein E2, T-cell surface glycoprotein E2
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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