Anti-CD99 Antibody [12E7 + MIC2/877] - BSA and Azide free (A252512)

Anti-CD99 Antibody [12E7 + MIC2/877] - BSA and Azide free

Mouse monoclonal [12E7 + MIC2/877] antibody to CD99.

This antibody recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 5-10 µl / million cells, IF: 1:100-1:200, IHC-P: 1:100-1:200

Human

Clone 12E7: Human acute lymphocytic leukemia T-cells. Clone MIC2/877: Recombinant human MIC2 protein.

Mouse

Oligoclonal

12E7 + MIC2/877

IgG1, kappa + IgG1, kappa

Protein A/G chromatography.

1 mg/ml

27-32 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

12E7, Antigen identified by monoclonal 12E7, Y homolog, Antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigen, CD99 molecule, CD99_HUMAN, Cell surface antigen 12E7, Cell surface antigen HBA 71, Cell surface antigen O13, E2 antigen, HBA71, MIC 2X, MIC 2Y, MIC2, MIC2 (monoclonal, MIC2X, MIC2Y, MSK5X, Protein MIC2, Surface antigen MIC2, T cell surface glycoprotein E2, T-cell surface glycoprotein E2