Mouse monoclonal [5E10] antibody to CD90/Thy1 (APC-Cyanine 7).
Specificity
This antibody recognizes CD90, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells, and neurons.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Porcine, Horse, Human, Non-Human Primates
Cross Reactivity
This antibody does not cross react with Dog.
Immunogen
HEL erythroleukemia cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
5E10
Isotype
IgG1
Light Chains
kappa
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of Jurkat cell suspension stained with Anti-CD90 Antibody [5E10] (APC-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
Separation of Jurkat cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis using Anti-CD90 Antibody [5E10] (APC-Cyanine 7) (4µl reagent per milion cells in 100µl of cell suspension).
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