This antibody recognises rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Reactivity

Rat

Immunogen

HTLV-1 transformed Lewis-S1 cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

24F

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Rat CD86 antibody, clone 24F recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages. Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production. Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maeda et al. 1997).

Synonyms

Activation B7-2 antigen, Activation B7-2 antigen 3, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, B7, B7 2, B7-2, B7.2, B70, B72 antigen, BU63, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD28LG2, CD86 antigen, CD86 antigen (CD28 antigen ligand 2, B7-2 antigen), CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2, CD86 molecule, CD86_HUMAN, CLS1, CTLA-4 counter-receptor B7.2, CTLA-4 counter-receptor B7.2 2, 3, Early T-cell costimulatory molecule 1, ETC-1, FUN 1, FUN-1, LAB72, Ly-58, Ly58, MB7, MB7-2, Membrane glycoprotein, MGC34413, T-lymphocyte activation antigen CD86, TS/A-2

Isotype Controls