This antibody recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa). The antibody does not react with formaldehyde-fixed cells; negative in western blotting.

Applications

Flow Cytometry, IP

Dilutions

Flow Cytometry: 1 µg/ml.

Reactivity

Human

Immunogen

Crude thymus membrane fraction.

Host

Mouse

Clonality

Monoclonal

Clone ID

MEM-31

Isotype

IgG2a

Conjugate

Biotin

Purification

This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.

Concentration

1 mg/ml

Predicted MW

32-34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store at 2-8°C. Do not freeze!

Synonyms

CD8 antigen, alpha polypeptide (p32), CD8a, CD8a antigen, CD8a molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, MAL, OKT8 T cell antigen, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen

Isotype Controls

Mouse IgG2a [MOPC-173] (Biotin) (A86310)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: Flow Cytometry - Anti-CD8 Antibody [MEM-31] (Biotin) (A85486)

Surface staining of human peripheral blood using Anti-CD8 Antibody (A85486).

Citations for this Clone (1)

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Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen.

References: Anti-CD8 Antibody [MEM-31] (Biotin) (ab28090)

Abstract:

Monoclonal antibodies against several human leucocyte cell surface antigens were prepared and characterized: B2M-02, a non-cytotoxic antibody against beta-2-microglobulin; MEM-09 and MEM-40, against immunoglobulin kappa-type light chains; MEM-12, MEM-24G and MEM-32B, all against a monomorphic determinant in MHC class II antigens, presumably HLA-DR, dependent on the association of alpha and beta chains; MEM-15 and MEM-18, against a monocyte antigen of 55 kDa; MEM-28, against a 200 kDa antigen expressed on all leucocytes; MEM-31, against the T8 antigen of the cytotoxic/suppressor T-lymphocyte subpopulation; MEM-32, against the T1 antigen of T lymphocytes.

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