This antibody recognises the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

Reactivity

Mouse

Immunogen

T cell clone, C6.

Host

Rat

Clonality

Monoclonal

Clone ID

KT15

Isotype

IgG2a

Conjugate

Excitation: 565nm, Emission: 578nm

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with 1 ml of distilled water.

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Rat anti Mouse CD8a, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

Synonyms

alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen

Isotype Controls