Anti-CD8 alpha Antibody [KT15] (APC) (A280825)

$205

Rat monoclonal [KT15] antibody to CD8 alpha (APC) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD8 alpha Antibody [KT15] (APC)
Description
Rat monoclonal [KT15] antibody to CD8 alpha (APC).
Specificity
This antibody recognises the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Reactivity
Mouse
Immunogen
T cell clone, C6.
Host
Rat
Clonality
Monoclonal
Clone ID
KT15
Isotype
IgG2a
Conjugate

APC

Excitation: 645nm, Emission: 660nm

Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD8a, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8a and CD8ß chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).
Synonyms
alpha polypeptide (p32), CD8, CD8 antigen alpha polypeptide, CD8 antigen alpha polypeptide (p32), CD8a, CD8A antigen, CD8A molecule, CD8A_HUMAN, Leu2, Leu2 T lymphocyte antigen, Ly3, LYT3, MAL, OKT8 T cell antigen, OTTHUMP00000160760, OTTHUMP00000160764, OTTHUMP00000203528, OTTHUMP00000203721, p32, T cell antigen Leu2, T cell co receptor, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T cell antigen, T8/Leu-2 T-lymphocyte differentiation antigen
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-CD8 alpha Antibody [KT15] (APC) (A280825)? Please let us know so that we can list the citation on this page.

Alternative products to Anti-CD8 alpha Antibody [KT15] (APC) (A280825)

Top