Recombinant Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588)

Name: Anti-CD7 Antibody [DM119] - BSA and Azide free
Brand: Antibodies.com
SKU: A318588
Price: 665.0 USD
Availability: InStock

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$665

Recombinant rabbit monoclonal [DM119] antibody to CD7 for ELISA and Flow Cytometry.

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Recombinant Antibody

Specifications

Name

Anti-CD7 Antibody [DM119] - BSA and Azide free

Description

Recombinant rabbit monoclonal [DM119] antibody to CD7.

Applications

ELISA, Flow Cytometry

Dilutions

ELISA: 1:5,000-10,000, Flow Cytometry: 1:100

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Clone ID

DM119

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity chromatography.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with distilled sterile water.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

General Notes

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyms

CD7 antigen, CD7 antigen (p41), CD7 molecule, CD7_HUMAN, GP40, LEU 9, LEU9, p41 protein, T cell antigen CD7, T cell leukemia antigen, T cell surface antigen Leu 9, T-cell antigen CD7, T-cell leukemia antigen, T-cell surface antigen Leu-9, Tp 40, Tp40, TP41

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588)

ELISA plates were pre-coated with Recombinant Human CD7 Protein (Fc Tag) (A318262) at 1 µg/ml (100 µl/well) which can bind Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588) in a linear range of 0.2-60 ng/ml.

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Figure 2: Flow Cytometry - Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588)

Flow cytometry analysis of Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588) on Expi293 cells transfected with human CD7 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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Figure 3: Flow Cytometry - Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588)

Flow cytometry analysis of antigen binding of Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588). (A) Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588) does not bind to 293T cells that do not express CD7. (B) A clear peak shift of Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588) was seen compared to the control when incubated with CD7-expressing Jurkat cells, indicating strong binding of Anti-CD7 Antibody [DM119] - BSA and Azide free (A318588) to CD7. Antibodies were incubated at 10 µg/ml. .

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