Anti-CD69 Antibody [FN50] (A280486)

Anti-CD69 Antibody [FN50]

Mouse monoclonal [FN50] antibody to CD69.

This antibody recognises the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel 1997), neutrophils, thymocytes and platelets. Expression of CD69 is rapidly induced on activation by infection or chronic inflamation. Multiple dimeric glycoforms of CD69 can be formed through differential glycosylation of the monomeric subunits. Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections.

Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human, Baboon, Chimpanzee, Cynomolgus monkey, Rhesus Monkey, Macaque

Activated human B-cells.

Mouse

Monoclonal

FN50

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD69 antibody, clone FN50 recognizes the human early activation antigen CD69, also known as activation inducer molecule (AIM), Early T-cell activation antigen p60, EA1 or MLR-3. CD69 is a 199 amino acid single pass type II transmembrane glycoprotein of ~30 kDa containing a single C-type lectin domain and a single potential N-glycosylation site. CD69 is expressed as a disulphide bond linked homodimer of ~60 kDa (López-Cabrera et al. 1993).CD69 is a marker of early activation expressed by B and T lymphocytes, natural killer cells(Werfel 1997), neutrophils, thymocytes and platelets (Gaviol et al. 1992). Expression of CD69 is rapidly induced on activation by infection or chronic inflamation (Sancho et al. 2005). Multiple dimeric glycoforms of CD69 can be formed through differential glycosylation of the monomeric subunits (Vance et al. 1997).Mouse anti Human CD69 , clone FN50 is useful for the detection of CD69 by flow cytometry and immunohistochemistry on frozen tissue sections.

Activation inducer molecule, Activation inducer molecule (AIM/CD69), AIM, BL-AC/P26, BLAC/P26, C-type lectin domain family 2 member C, CD69 antigen, CD69 antigen (p60, early T-cell activation antigen), CD69 molecule, CD69_HUMAN, CLEC2C, EA1, Early activation antigen CD69, Early lymphocyte activation antigen, Early T cell activation antigen p60, Early T-cell activation antigen p60, GP32/28, Leu23, Leukocyte surface antigen Leu-23, MLR-3, MLR3, VEA, Very Early Activation Antigen