This antibody recognises the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface. The expression of ED1 antigen being predominantly cytoplasmic , flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
Applications
Flow Cytometry
Dilutions
FC 1: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat, Bovine
Cross Reactivity
This antibody does not cross react with Horse
Immunogen
Rat spleen cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
ED1
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
Synonyms
CD 68, CD68 antigen, CD68 molecule, CD68_HUMAN, DKFZp686M18236, gp11, Gp110, LAMP4, MACROPHAGE ANTIGEN CD68, Macrophage antigen CD68 (microsialin), Macrosialin, SCARD1, Scavenger receptor class D member 1
