Anti-CD63 Antibody [NKI/C3] (CF® 488) (A251166)

Anti-CD63 Antibody [NKI/C3] (CF® 488)

Mouse monoclonal [NKI/C3] antibody to CD63 (CF® 488).

This antibody recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Flow Cytometry, IF

Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100

Human, Mouse

Smooth plasma membrane fraction of MeWo cells.

Mouse

Monoclonal

IgG1

kappa

CF® 488

Excitation: 490nm, Emission: 515nm

Protein A/G chromatography.

100 µg/ml

26 kDa (core protein) / 30-60 kDa (glycosylated)

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Store at 4-8°C. Antibody is stable for 24 months.

CD 63, CD63 antigen, CD63 antigen (melanoma 1 antigen), CD63 antigen melanoma 1 antigen, CD63 molecule, CD63_HUMAN, gp55, Granulophysin, LAMP 3, LAMP-3, LAMP3, LIMP, Lysosomal associated membrane protein 3, Lysosomal-associated membrane protein 3, Lysosome associated membrane glycoprotein 3, Mast cell antigen AD1, ME491, Melanoma 1 antigen, Melanoma associated antigen ME491, Melanoma associated antigen MLA1, Melanoma-associated antigen ME491, MGC72893, MLA 1, MLA1, NGA, Ocular melanoma associated antigen, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Tetraspanin 30, Tetraspanin-30, Tspan 30, Tspan-30, TSPAN30

This product is for research use only. It is not intended for diagnostic or therapeutic use.