Anti-CD62L Antibody [CC32] (A280039)

$515

Mouse monoclonal [CC32] antibody to CD62L for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD62L Antibody [CC32]
Description
Mouse monoclonal [CC32] antibody to CD62L.
Specificity
This antibody recognises bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor.Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive ?/d T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes. Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine, Sheep
Immunogen
Bovine lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CC32
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Bovine CD62L antibody, clone CC32 recognizes bovine L-selectin, also known as CD62L, Leukocyte-endothelial cell adhesion molecule 1, LECAM-1 or Lymph node homing receptor.Bovine CD62L is a 370 amino acid ~90 kDa, single pass type I transmembrane glycoprotein bearing a single C-type lectin domain, an EGF-like domain and two Sushi domains (UniProt: P98131). Immunoprecipitation of peripheral blood mononuclear cell lysates with Mouse anti Bovine CD62L antibody, clone CC32 reveals a molecule of ~90 kDa when run on polyacrylamide gels under reducing conditions, slightly larger than the murine and human CD62L homologues. Bovine CD62L is expressed on subpopulations of T-lymphocytes expressing CD2, CD4 and CD8. WC1 positive ?/d T cells also express CD62L as do a subpopulation of WC3+ve B-lymphocytes and all peripheral blood monocytes (Howard et al. 1992).Mouse anti Bovine CD62L antibody, clone CC32 has also been used successfully for the identification of CD62L on ovine peripheral blood cells by flow cytometry (Halliday et al. 2005).
Synonyms
A.11 , AI528707, CD62 antigen ligand , CD62 antigen-like family member L, gp90 MEL , gp90-MEL, hLHRc, IgA nephropathy, susceptibility to, included , L Selectin , L-selectin, LAM-1, LAM1 , LECAM1 , Leu8, Leukocyte adhesion molecule 1 , Leukocyte surface antigen Leu-8, Leukocyte surface antigen Leu8 , Leukocyte-endothelial cell adhesion molecule 1 , LNHR, LSEL, Ly-22 , Ly-m22 , Lyam-1, LYAM1 , LYAM1_HUMAN, Lymph node homing receptor , Lymphocyte adhesion molecule 1, Lymphocyte antigen 22 , Lymphocyte surface MEL-14 antigen , MEL-14, Pln homing receptor, PLNHR , Selectin L , Selectin, lymphocyte , SELL, TQ1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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