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Anti-NCAM1 Antibody (A82568)

Western Blot - Anti-NCAM1 Antibody (A82568) - Antibodies.com Immunohistochemistry - Anti-NCAM1 Antibody (A82568) - Antibodies.com Flow Cytometry - Anti-NCAM1 Antibody (A82568) - Antibodies.com
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for "NCAM Antibody"
$480
Product Datasheet

Goat polyclonal antibody to NCAM1 for ELISA, WB, IHC and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

Telephone
+1 (314) 370-6046
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Email
orders@antibodies.com

Name
Anti-NCAM1 Antibody
Description
Goat polyclonal antibody to NCAM1.
Applications
ELISA, WB, IHC, Flow Cytometry
Dilutions
ELISA: 1:32,000, WB: 0.03-0.3µg/ml, IHC: 5µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region of human NCAM1 (Isoform 2).
Sequence
C-KASWTRPEKQETLD
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
150-170 kDa
Predicted MW
94.6kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
antigen MSK39 identified by monoclonal, antigen recognized by monoclonal, CD56, cell adhesion molecule, neural, 1, MSK 39, MSK39, N-CAM-1, NCAM, NCAM 1, NCAM C, NCAM-1, NCAM1_HUMAN, NCAMC, Neural cell adhesion molecule 1, Neural cell adhesion molecule NCAM, OTTHUMP00000235666
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-NCAM1 Antibody (A82568) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-NCAM1 Antibody (A82568)
NCAM1 expression in Human Frontal Cortex (A) and Cerebellum (B) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-NCAM1 Antibody (A82568) at 0.01µg/ml (A) or 0.1µg/ml (B) and detected by chemiluminescence.
Immunohistochemistry - Anti-NCAM1 Antibody (A82568) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-NCAM1 Antibody (A82568)
NCAM1 expression in Human Adrenal Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-NCAM1 Antibody (A82568) at 5µg/ml and revealed with alkaline phosphatase (AP).
Flow Cytometry - Anti-NCAM1 Antibody (A82568) - Antibodies.comEnlarge Image
Figure 3: Flow Cytometry - Anti-NCAM1 Antibody (A82568)
NCAM1 expression in U251 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-NCAM1 Antibody (A82568) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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