This antibody reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most thymocytes, and B lymphocytes subset (B-1a lymphocytes).

Applications

Flow Cytometry

Dilutions

Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

Human acute lymphoblastic leukemia (ALL) T cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

L17F12

Isotype

IgG2a

Light Chains

kappa

Conjugate

PerCP

Excitation: 482nm, Emission: 675nm

Purification

This antibody is conjugated with PerCP under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

Predicted MW

67 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Storage

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

Synonyms

CD 5, CD5 antigen, CD5 antigen (p56 62), CD5 molecule, CD5_HUMAN, LEU1, LyA, Lymphocyte antigen T1/Leu-1, OTTHUMP00000236973, T-cell surface glycoprotein CD5, T1

Isotype Controls