This antibody recognises the equine CD5 antigen, a single-pass type I membrane protein with an approximate molecular weight of 69 kDa. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Horse
Immunogen
Equine leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CVS5
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Horse CD5 antibody, clone CVS5 recognizes the equine CD5 antigen, a single-pass type I membrane protein with an approximate molecular weight of 69 kDa. Equine CD5 is expressed on the majority of T-lymphocytes, in addition is has been reported that equine CD5 may also be detected at very low levels on B-cells and granulocyctes.In addition to the CVS5 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
