Mouse monoclonal [HP2/1] antibody to Integrin alpha 4 / CD49D.
Specificity
This antibody recognises human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria. Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells. Mouse anti Human CD49d, clone HP2/1 can be used in assays of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and inhibits cell binding to soluble VCAM-1.
Supplied in Phosphate Buffered Saline with 0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD49d monoclonal antibody, clone HP2/1 recognizes human CD49d also known as integrin alpha-4 or VLA-4 subunit alpha. CD49d is a ~150kDa single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kDa (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form the Peyer's patch-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer’s patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997).Mouse anti human CD49d, clone HP2/1 binds to both intact and the 80kDa fragment of integrin alpha-4. CD49d is expressed on monocytes, T cells, B cells, thymocytes and Langerhans cells (de Graaf et al. 1995).Mouse anti Human CD49d, clone HP2/1 can be used in assays of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and inhibits cell binding to soluble VCAM-1 (Weller et al. 1991).
