Anti-CD48 Antibody [ARC53030] (A308320)

Name: Anti-CD48 Antibody [ARC53030]
Brand: Antibodies.com
SKU: A308320
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC53030] antibody to CD48 for WB and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-CD48 Antibody [ARC53030]

Description

Rabbit monoclonal [ARC53030] antibody to CD48.

Applications

WB, Flow Cytometry

Dilutions

WB: 1:2,000-1:20,000, Flow Cytometry: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-220 of human CD48 (NP_001769.2).

Sequence

QGHLVHMTVVSGSNVTLNISESLPENYKQLTWFYTFDQKIVEWDSRKSKYFESKFKGRVRLDPQSGALYISKVQKEDNSTYIMRVLKKTGNEQEWKIKLQVLDPVPKPVIKIEKIEDMDDNCYLKLSCVIPGESVNYTWYGDKRPFPKELQNSVLETTLMPHNYSRCYTCQVSNSVSSKNGTVCLSPPCTLARS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC53030

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

46 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen CD48, B cell membrane protein, B lymphocyte activation marker BLAST 1, B-cell activation marker, B-lymphocyte activation marker BLAST-1, BCM 1 surface antigen, BCM1, BCM1 surface antigen, BLAST, BLAST 1, BLAST1, CD 48, CD48 antigen, CD48 antigen (B cell membrane protein), CD48 molecule, CD48 protein, CD48_HUMAN, hCD48, Leucocyte antigen MEM 102, Leukocyte antigen MEM-102, mCD48, MEM 102, MEM-102, MEM102, Signaling lymphocytic activation molecule 2, SLAM family member 2, SLAMF 2, SLAMF2, TCT.1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CD48 Antibody [ARC53030] (A308320)

Western blot analysis of various lysates, using Anti-CD48 Antibody [ARC53030] (A308320) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Flow Cytometry - Anti-CD48 Antibody [ARC53030] (A308320)

Flow cytometry analysis of Daudi cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD48 Antibody [ARC53030] (A308320), (10 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained Daudi cells, used as blank control (red line).

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Figure 3: Flow Cytometry - Anti-CD48 Antibody [ARC53030] (A308320)

Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-CD48 Antibody [ARC53030] (A308320), (10 µg/ml orange line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained Raji cells, used as blank control (red line).

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