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Anti-CD47 Antibody (A82915)

Immunofluorescence - Anti-CD47 Antibody (A82915) - Antibodies.com Immunofluorescence - Anti-CD47 Antibody (A82915) - Antibodies.com Immunohistochemistry - Anti-CD47 Antibody (A82915) - Antibodies.com Immunohistochemistry - Anti-CD47 Antibody (A82915) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to CD47 for ELISA, IF and IHC.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD47 Antibody
Description
Goat polyclonal antibody to CD47.
Applications
ELISA, IF, IHC
Dilutions
ELISA: 1:128,000, IHC: 6-7µg/ml, IF: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the internal region (near C terminus) of human CD47.
Sequence
C-SNQKTIQPPRK
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Antigen identified by monoclonal, Antigenic surface determinant protein OA3, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-CD47 Antibody (A82915) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-CD47 Antibody (A82915)
CD47 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CD47 Antibody (A82915) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Immunofluorescence - Anti-CD47 Antibody (A82915) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CD47 Antibody (A82915)
CD47 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-CD47 Antibody (A82915) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, plasma membrane, and some nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Immunohistochemistry - Anti-CD47 Antibody (A82915) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD47 Antibody (A82915)
CD47 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-CD47 Antibody (A82915) at 7µg/ml and revealed with horseradish peroxidase (HRP).
Immunohistochemistry - Anti-CD47 Antibody (A82915) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD47 Antibody (A82915)
Negative control for CD47 expression in Human Cortex analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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