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Anti-CD47 Antibody (A15622)

Western Blot - Anti-CD47 Antibody (A15622) - Antibodies.com Immunohistochemistry - Anti-CD47 Antibody (A15622) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD47 for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-CD47 Antibody
Description
Rabbit polyclonal antibody to CD47.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 224-323 of human CD47 (NP_001768.1).
Sequence
HYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 - 50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Antigen identified by monoclonal, Antigenic surface determinant protein OA3, CD 47, CD47 antigen, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 glycoprotein, CD47 molecule, CD47_HUMAN, IAP, Integrin Associated Protein, Integrin associated signal transducer, Integrin-associated protein, Leukocyte surface antigen CD47, MER 6, MER6, OA 3, OA3, OTTHUMP00000041152, OTTHUMP00000041153, Protein MER6, Rh related antigen, Surface antigen identified by monoclonal
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD47 Antibody (A15622) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD47 Antibody (A15622)
Western blot analysis of extracts of various cell lines, using Anti-CD47 Antibody (A15622) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry - Anti-CD47 Antibody (A15622) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD47 Antibody (A15622)
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CD47 Antibody (A15622) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Publishing research using Anti-CD47 Antibody (A15622)? Please let us know so that we can list the citation on this page.

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