Anti-CD47 Antibody [1/1A4] (PE) (A320256)

Anti-CD47 Antibody [1/1A4] (PE)

Mouse monoclonal [1/1A4] antibody to CD47 (PE).

This antibody recognises human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1. Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

Human erythrocytes.

Mouse

Monoclonal

1/1A4

IgG2b

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD47 antibody, clone 1/1A4 recognizes human CD47, also known as integrin-associated protein, a 50kDa glycoprotein expressed on all haematopoietic cell lines, epithelial cells, endothelial cells, fibroblasts and many tumour cell lines. CD47 is involved in several cellular processes including regulation of T cell and dendritic cell activation, regulation of neutrophil (PMN) chemotaxis, caspase-independent apoptosis, and may act as a signal transducer in the regulation of cation fluxes across cell membranes. CD47 has been identified as a ligand for the inhibitory macrophage receptor known as signal regulatory protein alpha (SIRPalpha) and as a receptor for thrombospondin-1. Cross-linking of CD47 with clone 1/1A4 co-stimulates the proliferation of T cells (Waclavicek et al. 1993).

Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6

This product is for research use only. It is not intended for diagnostic or therapeutic use.