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Anti-CD46 Antibody [ARC0428] (A307262)

Western Blot - Anti-CD46 Antibody [ARC0428] (A307262) - Antibodies.com Immunohistochemistry - Anti-CD46 Antibody [ARC0428] (A307262) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0428] antibody to CD46 for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-CD46 Antibody [ARC0428]
Description
Rabbit monoclonal [ARC0428] antibody to CD46.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD46 (P15529).
Sequence
MEPPGRRECPFPSWRFPGLLLAAMVLLLYSFSDACEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0428
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 - 75 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AHUS2, Antigen defined by monoclonal, Antigen identified by monoclonal, CD46 antigen, CD46 antigen complement regulatory protein, CD46 molecule, CD46 molecule complement regulatory protein, Complement membrane cofactor protein, MCP, MCP_HUMAN, Measles virus receptor, Membrane cofactor protein, membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen), MGC26544, MIC10, TLX, TRA2.10, Trophoblast leucocyte common antigen, Trophoblast leukocyte common antigen, Trophoblast lymphocyte cross reactive antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD46 Antibody [ARC0428] (A307262) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD46 Antibody [ARC0428] (A307262)
Western blot analysis of extracts of various cell lines, using Anti-CD46 Antibody [ARC0428] (A307262) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-CD46 Antibody [ARC0428] (A307262) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD46 Antibody [ARC0428] (A307262)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CD46 Antibody [ARC0428] (A307262) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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