Anti-CD45RO Antibody [IL-A116] (PE) (A280712)

Anti-CD45RO Antibody [IL-A116] (PE)

Mouse monoclonal [IL-A116] antibody to CD45RO (PE).

This antibody recognises the bovine homologue of the human CD45RO cell surface antigen.CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years. recognises the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine, Goat

Bovine peripheral blood monouclear cells.

Mouse

Monoclonal

IL-A116

IgG3

PE

Excitation: 565nm, Emission: 578nm

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Bovine CD45RO, clone IL-A116 recognizes the bovine homologue of the human CD45RO cell surface antigen.CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). Mouse anti Bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995), analogus to the molecular weight of human and mouse CD45RO.Mouse anti Bovine CD45RO, clone IL-A116 recognizes the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995).

B220, CD45, cd45 antigen, CD45R, GP180, L-CA, lca, Leukocyte common antigen, LY 5, LY5, Protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

This product is for research use only. It is not intended for diagnostic or therapeutic use.