Anti-CD45RO Antibody [IL-A116] (A280713)

Anti-CD45RO Antibody [IL-A116]

Mouse monoclonal [IL-A116] antibody to CD45RO.

This antibody recognises the bovine homologue of the human CD45RO cell surface antigen.CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years. recognises the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus.

IP, Flow Cytometry

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine, Goat

Bovine peripheral blood monouclear cells.

Mouse

Monoclonal

IL-A116

IgG3

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at 4°C.

Mouse anti Bovine CD45RO, clone IL-A116 recognizes the bovine homologue of the human CD45RO cell surface antigen.CD45, also known as Leucocyte Common Antigen or LCA, occurs in a number of isoforms, clone IL-A116 is specific for the low molecular weight isoform termed CD45RO, the isoform associated with expression on memory T-cells. Bovine CD45RO is expressed by monocytes, granulocytes and subsets of thymocytes, CD4+ T cells and CD8+ T cells. CD45RO+ CD8+ T cells increase from approximately 5% in neonatal calves to approximately 35% in adult cattle over the age of 5 years (Hogg et al. 2011). Mouse anti Bovine CD45RO, clone IL-A116 immunoprecipitates a molecule of ~180 kDa (Bembridge et al. 1995), analogus to the molecular weight of human and mouse CD45RO.Mouse anti Bovine CD45RO, clone IL-A116 recognizes the CD45RO cell surface antigen by flow cytometry in both European cattle, Bos taurus, and in Zebu, B.indicus (Bembridge et al. 1995).

B220, CD45, cd45 antigen, CD45R, GP180, L-CA, lca, Leukocyte common antigen, LY 5, LY5, Protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen