This antibody recognises the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes. The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Bovine

Immunogen

Bovine thymocytes.

Host

Mouse

Clonality

Monoclonal

Clone ID

CC76

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Bovine CD45RB, clone CC76, recognizes the RB isoform of the bovine leucocyte common antigen expressed on subpopulations of CD4+ and CD8+ T cells. Expression levels of CD45RB are used as a marker for differentiating naïve and memory T lymphocytes (Howard et al. 1991). The antigen recognized by clone CC76 is also expressed on B cells but is absent from both macrophages and granulocytes.

Synonyms

B220, CD 45, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, LY5, Lyt4, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

Isotype Controls