This antibody recognises the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 100µl lysed whole blood

Reactivity

Human, Rhesus Monkey

Immunogen

Human T lymphocytes.

Host

Mouse

Clonality

Monoclonal

Clone ID

F8-11-13

Isotype

IgG1

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Mouse anti Human CD45RA antibody, clone F8-11-13 recognizes the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc.Mouse anti Human CD45RA antibody, clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes.Mouse anti Human CD45RA antibody, clone F8-11-13 is routinely tested in flow cytometry on human peripheral blood lymphocytes.

Synonyms

B220, CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, L-CA, LCA, Leukocyte common antigen, Loc, Ly-5, LY5, Ly5, homolog of, Lymphocyte antigen 5, Lyt-4, Protein tyrosine phosphatase, receptor type C, Protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, RT7, T200, T200 glycoprotein, T200 leukocyte common antigen

Isotype Controls