This antibody recognises canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts,. It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1 x 10⁶ cells in 100µl

Reactivity

Canine

Immunogen

Canine thymocytes.

Host

Rat

Clonality

Monoclonal

Clone ID

YKIX753.22.2

Isotype

IgG2b

Conjugate

Excitation: 565nm, Emission: 578nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with 1 ml of distilled water

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Storage

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Rat anti Dog CD45R antibody, clone YKIX753.22.2 recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobbold et al. 1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13 (Cobbold et al. 1994).

Synonyms

B220, CD45, CD45 antigen, Glycoprotein, GP180, L CA, L-CA, LCA, Leukocyte common antigen, Leukocyte common antigen precursor, loc, Ly-5, LY5, Lyt-4, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, Ptprc, PTPRC_HUMAN, Receptor type tyrosine protein phosphatase C, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

Isotype Controls