This antibody recognises mouse CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa). Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Reactivity

Mouse, Human, Cat

Immunogen

Murine leukemia-induced pre-B tumor cells (RAW112).

Host

Rat

Clonality

Monoclonal

Clone ID

RA3-6B2

Isotype

IgG2a

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Rat anti Mouse CD45R antibody, clone RA3-6B2 recognizes murine CD45R, a form of the CD45 antigen expressed by B cells and lytically active subsets of NK cells and non-MHC restricted CTL's. Rat anti Mouse CD45R antibody, clone RA3-6B2 immunoprecipitates the high molecular weight form of CD45 (220 kDa).Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).

Synonyms

B220, CD45, CD45 antigen, Glycoprotein, GP180, L CA, L-CA, LCA, Leukocyte common antigen, Leukocyte common antigen precursor, loc, Ly-5, LY5, Lyt-4, Protein tyrosine phosphatase receptor type C, Protein tyrosine phosphatase receptor type c polypeptide, Ptprc, PTPRC_HUMAN, Receptor type tyrosine protein phosphatase C, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen

Isotype Controls