Anti-CD45 Antibody [F10-89-4] (Amethyst Orange) (A280399)

$515

Mouse monoclonal [F10-89-4] antibody to CD45 (Amethyst Orange) for Flow Cytometry.

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD45 Antibody [F10-89-4] (Amethyst Orange)
Description
Mouse monoclonal [F10-89-4] antibody to CD45 (Amethyst Orange).
Specificity
This antibody recognises the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:50, IHC-Fr: 1:500 - 1:1,000, IHC-P: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
F10-89-4
Isotype
IgG2a
Conjugate

Amethyst Orange

Excitation: 390nm, Emission: 535nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Synonyms
B220, CD 45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, loc, Ly-5, LY5, Ly5, homolog of, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, Protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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