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Anti-CD45 Antibody [ARC0459] (A308608)

Western Blot - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.com Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.com Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.com Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0459] antibody to CD45 for WB and IHC.

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Anti-CD45 Antibody [2D1]
Mouse monoclonal [2D1] antibody to CD45 for Flow Cytometry.
Name
Anti-CD45 Antibody [ARC0459]
Description
Rabbit monoclonal [ARC0459] antibody to CD45.
Applications
WB, IHC
Dilutions
WB: 1:50-1:200, IHC: 1:50-1:200
Reactivity
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 194-390 of human CD45 (NP_002829.3).
Sequence
SDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFG
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0459
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
180 - 250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
B220, CD 45, CD45 antigen, CD45R, GP180, L-CA, LCA, Leukocyte common antigen, loc, Ly-5, LY5, Ly5, homolog of, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyrosine phosphatase receptor type c polypeptide, Protein tyrosine phosphatase, receptor type C, protein tyrosine phosphatase, receptor type, C, Protein tyrosine phosphatase, receptor type, c polypeptide, Ptprc, PTPRC_HUMAN, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CD45 Antibody [ARC0459] (A308608)
Western blot analysis of extracts of various cell lines, using Anti-CD45 Antibody [ARC0459] (A308608) at 1:200 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608)
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-CD45 Antibody [ARC0459] (A308608)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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