Anti-CD43 Antibody [W3/13] (A281950)

$340

Mouse monoclonal [W3/13] antibody to CD43 for IHC-P, Flow Cytometry and IHC-Fr.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-CD43 Antibody [W3/13]
Description
Mouse monoclonal [W3/13] antibody to CD43.
Specificity
This antibody recognises the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
Applications
IHC-P, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:50 for 1 mg, 0.1 mg, 1:25 for 0.25 mg, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
Rat Thymocyte Membrane Glycoproteins.
Host
Mouse
Clonality
Monoclonal
Clone ID
W3/13
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.
Synonyms
CD 43, CD43 antigen, Galactoglycoprotein, GALGP, GPL 115, GPL115, Human gene for sialophorin, Leucocyte sialoglycoprotein, Leukocyte large sialoglycoprotein, Leukocyte sialoglycoprotein, Leukosialin, LEUK_HUMAN, LSN, Ly-48, Sialophorin, sialophorin (gpL115, leukosialin, CD43), Spn
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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