Anti-CD43 Antibody [DFT-1] (A280250)

Anti-CD43 Antibody [DFT-1]

Mouse monoclonal [DFT-1] antibody to CD43.

This antibody recognises human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a ~115 kDa form present on all T-cells and a ~130 kDa form indicative of T-cell activation and acts as a T-cell counter-receptor for CD169/Siglec-1. Mouse anti Human CD43 antibody, clone DFT-1 binds to both glycoforms of human CD43 CD34 expression is deficient and/or defective in patients suffering from the X-linked chromosomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54. Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes.

IHC-P, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:100, IHC-P: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. This product does not require protein digestion pre-treatment of paraffin sections however, staining of paraffin embedded formalin fixed tissues may be improved in some cases by enzyme pretreatment using Trypsin.

Human

KG-1 cells.

Mouse

Monoclonal

DFT-1

IgG1

Unconjugated

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin. CD43 is a 381 amino acid single pass type I transmembrane glycoprotein present on the cell surface of multiple cell types including thymocytes, T-lymphocytes, neutrophils, plasma cells and platelets. It is lacking on the surface of most B-lymphocytes. CD34 is a major membrane sialoglycoprotein expressed on T lymphocytes as two predominant glycoforms, a ~115 kDa form present on all T-cells and a ~130 kDa form indicative of T-cell activation (Barren et al. 1997) and acts as a T-cell counter-receptor for CD169/Siglec-1 (van den Berg et al. 2001). Mouse anti Human CD43 antibody, clone DFT-1 binds to both glycoforms of human CD43 (Kyoizumi et al. 2004)CD34 expression is deficient and/or defective in patients suffering from the X-linked chromosomal immunodeficiency condition Wiskott-Aldrich syndrome and acts as a ligand for CD54 (Rosenstein et al. 1991). Mouse anti human CD43, clone DFT-1 has been used successfully for the evaluation of CD43 expression in patients with Myelodysplastic syndromes (Kyriakou et al. 2006).

CD 43, CD43 antigen, Galactoglycoprotein, GALGP, GPL 115, GPL115, Human gene for sialophorin, Leucocyte sialoglycoprotein, Leukocyte large sialoglycoprotein, Leukocyte sialoglycoprotein, Leukosialin, LEUK_HUMAN, LSN, Ly-48, Sialophorin, sialophorin (gpL115, leukosialin, CD43), Spn