Anti-CD4 Antibody [RPA-T4] (A281707)

Anti-CD4 Antibody [RPA-T4]

Mouse monoclonal [RPA-T4] antibody to CD4.

This antibody recognises human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule. Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, IHC-Fr 1: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Human

Human PHA blasts.

Mouse

Monoclonal

RPA-T4

IgG1

Unconjugated

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti human CD4 antibody, clone RPA-T4 recognizes human CD4, a ~55 kDa cell surface glycoprotein, primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Epitope mapping shows that antibodies, produced by clone RPA-T4, recognize an epitope within domain 1 of the extracellular region of the CD4 molecule.Mouse anti human CD4 antibody, clone RPA-T4 blocks gp120-CD4 interaction and inhibits syncytium formation (Piatier-Tonneau et al, 1997). The use of Mouse anti Human CD4:Low Endotoxin (MCA1267EL) is recommended for functional assays.

CD 4, CD4 (L3T4), CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4+ Lymphocyte deficiency, included, CD4mut, CD4_HUMAN, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, OTTHUMP00000238897, p55, T cell antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, included, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, W3/25, W3/25 antigen