This antibody recognises mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: Neat - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Reactivity

Mouse

Immunogen

Murine CD4.

Host

Rat

Clonality

Monoclonal

Clone ID

GK1.5

Isotype

IgG2b

Conjugate

FITC

Excitation: 490nm, Emission: 525nm

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

100 µg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Storage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

General Notes

Rat anti Mouse CD4 antibody, clone GK1.5 recognizes mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells.

Synonyms

CD 4, CD4 (L3T4), CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4+ Lymphocyte deficiency, included, CD4mut, CD4_HUMAN, L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, MGC165891, OTTHUMP00000238897, p55, T cell antigen T4, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T cell OKT4 deficiency, included, T cell surface antigen T4/Leu 3, T cell surface antigen T4/Leu3, T cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, W3/25, W3/25 antigen

Isotype Controls