Anti-CD3D Antibody (A16813)

Name: Anti-CD3D Antibody
Brand: Antibodies.com
SKU: A16813
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CD3D for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CD3D Antibody

Description

Rabbit polyclonal antibody to CD3D.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-105 of human CD3D (NP_000723.1).

Sequence

FKIPIEELEDRVFVNCNTSITWVEGTVGTLLSDITRLDLGKRILDPRGIYRCNGTDIYKDKESTVQVHYRMCQSCVELDPATVA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

19 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CD3 antigen delta subunit, CD3 delta, CD3-delta, CD3d antigen delta polypeptide (TiT3 complex), CD3d molecule, CD3d molecule delta (CD3-TCR complex), CD3D_HUMAN, IMD19, OKT3 delta chain, T cell receptor T3 delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-CD3D Antibody (A16813)

Western blot analysis of extracts of Jurkat cells, using Anti-CD3D Antibody (A16813) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-CD3D Antibody (A16813)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-CD3D Antibody (A16813) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.