Mouse monoclonal [QBEnd-10] antibody to CD34 (APC).
Specificity
This antibody recognizes an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Non-Human Primates, Human
Cross Reactivity
Dog, Sheep, Rat, Cow
Immunogen
Human endothelial vesicles.
Host
Mouse
Clonality
Monoclonal
Clone ID
QBEnd-10
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
Purified antibody is conjugated with APC under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!