Mouse monoclonal [4H11[APG]] antibody to CD34 (Biotin).
Specificity
This antibody reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells. It is gradually lost on progenitor cells. This antibody completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Applications
Flow Cytometry, WB, IHC-P, ICC
Dilutions
Flow Cytometry: 1-2 µg/ml.
Reactivity
Human
Immunogen
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
4H11[APG
Isotype
IgG1
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
110-115 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
