Anti-CD32 Antibody [AT10] (A279687)

Anti-CD32 Antibody [AT10]

Mouse monoclonal [AT10] antibody to CD32.

This antibody recognises the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII.

IP, Flow Cytometry, IHC-Fr, Functional Assays

Flow Cytometry: 20 µg/ml, IHC-Fr 2: 1:500 - 1:1,000, IP: 20 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells or cells or 100µl whole blood

Human, Dog, Rhesus Monkey, Pig

K562 cell line.

Mouse

Monoclonal

AT10

IgG1

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in TRIS Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD32 antibody, clone AT10 recognizes the human CD32 antigen, a ~40 kDa glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). CD32 mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets.Mouse anti Human CD32 antibody, clone AT10 blocks the binding of IgG to Fc gamma RII (Larsson et al. 1997).

8D6 antigen, 8D6A, CD320, CD320 antigen, CDw32, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, Fc-gamma-RIIb, Fc-gamma-RIIc, FCG2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, FcRII-a, FcRII-b, FcRII-c, FDC-signaling molecule 8D6, FDC-SM-8D6, IGFR2, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, TCblR, Transcobalamin receptor