Anti-CD32 Antibody [7.3] (A248533)

Anti-CD32 Antibody [7.3]

Mouse monoclonal [7.3] antibody to CD32.

This antibody reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

Functional Studies, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

K562 and FcgRII+L cells.

Mouse

Monoclonal

7.3

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

40 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD32 Antibody [7.3] - BSA and Azide free (A251715).

8D6 antigen, 8D6A, CD320, CD320 antigen, CDw32, Fc-gamma RII-a, Fc-gamma RII-b, Fc-gamma RII-c, Fc-gamma-RIIa, Fc-gamma-RIIb, Fc-gamma-RIIc, FCG2, FCGR2A, FCGR2A1, FCGR2B, FCGR2C, FcRII-a, FcRII-b, FcRII-c, FDC-signaling molecule 8D6, FDC-SM-8D6, IGFR2, IgG Fc receptor II-a, IgG Fc receptor II-b, IgG Fc receptor II-c, Low affinity immunoglobulin gamma Fc region receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-b, Low affinity immunoglobulin gamma Fc region receptor II-c, TCblR, Transcobalamin receptor