Anti-CD31 Antibody [TLD-3A12] - BSA and Azide free (A281834)

Overview
Specifications

Mouse monoclonal [TLD-3A12] antibody to CD31 for Flow Cytometry, WB, ELISA, IHC-Fr and IF.

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Alternative Formats and ConjugatesAlternative Formats

Biotin FITC PE Unconjugated

Delivery

Freight Charges

$40 to United States

Lead Time

4-6 Business Days

Contact Us

Telephone

+1 (314) 370-6046

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Tags:

Embryonic Stem Cell Marker Endothelial Stem Cell Marker Hematopoietic Stem Cell Marker Intercellular Junction Marker

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Specifications

Name

Anti-CD31 Antibody [TLD-3A12] - BSA and Azide free

Description

Mouse monoclonal [TLD-3A12] antibody to CD31.

Specificity

This antibody recognises rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque

Applications

Flow Cytometry, WB, ELISA, IHC-Fr, IF

Dilutions

Flow Cytometry: 1:10 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Reactivity

Rat, Rhesus Monkey, Pig

Immunogen

Activated, Lewis rat derived microglial cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

TLD-3A12

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G affinity chromatography of tissue culture supernatant.

Concentration

1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline.

Storage

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

General Notes

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)

Synonyms

Adhesion molecule, CD31 antigen, CD31 EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA, GPIIA', GPIIA'', HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM, PECAM 1, PECAM 1 CD31 EndoCAM, PECAM-1, Pecam1, Platelet and endothelial cell adhesion molecule 1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

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